Construct: shRNA TRCN0000219077

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006696.3-2583s21c1
DNA Barcode:
CTGAATTTCAGCGTGACATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRD8 (10902)
Gene Description:
bromodomain containing 8
Transcript:
RefSeq NM_006696.3 (NON-CURRENT)
Match location:
Position 2583 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219077)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAATTTCAGCGTGACATTA
Hairpin Sequence:
5'-CCGG-CTGAATTTCAGCGTGACATTA-CTCGAG-TAATGTCACGCTGAAATTCAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAATTTCAGCGTGACATTACTCGAGTAATGTCACGCTGAAATTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAATTTCAGCGTGACATTACTCGAGTAATGTCACGCTGAAATTCAG-3'

Other clones with same target sequence:

(none)