Construct: shRNA TRCN0000219092

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_985507.1-218s21c1
DNA Barcode:
ACTGCTATAGGCTACAATTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
EG666702 (666702)
Gene Description:
predicted gene, EG666702
Transcript:
RefSeq XM_985507.1 (NON-CURRENT)
Match location:
Position 218 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000219092)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGCTATAGGCTACAATTTG
Hairpin Sequence:
5'-CCGG-ACTGCTATAGGCTACAATTTG-CTCGAG-CAAATTGTAGCCTATAGCAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGCTATAGGCTACAATTTGCTCGAGCAAATTGTAGCCTATAGCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGCTATAGGCTACAATTTGCTCGAGCAAATTGTAGCCTATAGCAGT-3'

Other clones with same target sequence:

(none)