Construct: shRNA TRCN0000225615

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_912467.2-736s21c1
DNA Barcode:
ACACAGTATTCAGATCTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp813-ps (636910)
Gene Description:
zinc finger protein 813, pseudogene
Transcript:
RefSeq XM_912467.2 (NON-CURRENT)
Match location:
Position 736 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000225615)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACAGTATTCAGATCTTAAA
Hairpin Sequence:
5'-CCGG-ACACAGTATTCAGATCTTAAA-CTCGAG-TTTAAGATCTGAATACTGTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACAGTATTCAGATCTTAAACTCGAGTTTAAGATCTGAATACTGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACAGTATTCAGATCTTAAACTCGAGTTTAAGATCTGAATACTGTGT-3'

Other clones with same target sequence:

(none)