Construct: shRNA TRCN0000225633

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_914267.2-1859s21c1
DNA Barcode:
TTCATGAGCACAACAAGATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp651 (270210)
Gene Description:
zinc finger protein 651
Transcript:
RefSeq XM_914267.2 (NON-CURRENT)
Match location:
Position 1859 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000225633)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCATGAGCACAACAAGATAG
Hairpin Sequence:
5'-CCGG-TTCATGAGCACAACAAGATAG-CTCGAG-CTATCTTGTTGTGCTCATGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCATGAGCACAACAAGATAGCTCGAGCTATCTTGTTGTGCTCATGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCATGAGCACAACAAGATAGCTCGAGCTATCTTGTTGTGCTCATGAA-3'

Other clones with same target sequence:

(none)