Construct: shRNA TRCN0000225645

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_356228.4-1247s21c1
DNA Barcode:
CCTCGCTCACTGGACATAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp558 (72230)
Gene Description:
zinc finger protein 558
Transcript:
RefSeq XM_356228.4 (NON-CURRENT)
Match location:
Position 1247 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000225645)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGCTCACTGGACATAATA
Hairpin Sequence:
5'-CCGG-CCTCGCTCACTGGACATAATA-CTCGAG-TATTATGTCCAGTGAGCGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGCTCACTGGACATAATACTCGAGTATTATGTCCAGTGAGCGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGCTCACTGGACATAATACTCGAGTATTATGTCCAGTGAGCGAGG-3'

Other clones with same target sequence:

(none)