Construct: shRNA TRCN0000225681

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_885170.2-203s21c1
DNA Barcode:
CTCCGCTGTTACACCATTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpr52 (620246)
Gene Description:
G protein-coupled receptor 52
Transcript:
RefSeq XM_885170.2 (NON-CURRENT)
Match location:
Position 203 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000225681)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCGCTGTTACACCATTATA
Hairpin Sequence:
5'-CCGG-CTCCGCTGTTACACCATTATA-CTCGAG-TATAATGGTGTAACAGCGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCGCTGTTACACCATTATACTCGAGTATAATGGTGTAACAGCGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCGCTGTTACACCATTATACTCGAGTATAATGGTGTAACAGCGGAG-3'

Other clones with same target sequence:

(none)