Construct: shRNA TRCN0000225733

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175251.2-2425s21c1
DNA Barcode:
ACCTGTCCCGCCTACTAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arid2 (77044)
Gene Description:
AT rich interactive domain 2 (ARID, RFX-like)
Transcript:
RefSeq NM_175251.2 (NON-CURRENT)
Match location:
Position 2425 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000225733)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTGTCCCGCCTACTAATAA
Hairpin Sequence:
5'-CCGG-ACCTGTCCCGCCTACTAATAA-CTCGAG-TTATTAGTAGGCGGGACAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTGTCCCGCCTACTAATAACTCGAGTTATTAGTAGGCGGGACAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTGTCCCGCCTACTAATAACTCGAGTTATTAGTAGGCGGGACAGGT-3'

Other clones with same target sequence:

(none)