Construct: shRNA TRCN0000225742

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008641.2-2271s21c1
DNA Barcode:
ACTTGTATGAGGGTCATATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mast2 (17776)
Gene Description:
microtubule associated serine/threonine kinase 2
Transcript:
RefSeq NM_008641.2 (NON-CURRENT)
Match location:
Position 2271 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000225742)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTGTATGAGGGTCATATTG
Hairpin Sequence:
5'-CCGG-ACTTGTATGAGGGTCATATTG-CTCGAG-CAATATGACCCTCATACAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTGTATGAGGGTCATATTGCTCGAGCAATATGACCCTCATACAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTGTATGAGGGTCATATTGCTCGAGCAATATGACCCTCATACAAGT-3'

Other clones with same target sequence:

  1. TRCN0000333014
  2. TRCN0000219732