Construct: shRNA TRCN0000225763

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028017.1-1950s21c1
DNA Barcode:
GCCCTTGTGTGTGACTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Napg (108123)
Gene Description:
N-ethylmaleimide sensitive fusion protein attachment protein gamma
Transcript:
RefSeq NM_028017.1 (CURRENT)
Match location:
Position 1950 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000225763)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTGTGTGTGACTATAAA
Hairpin Sequence:
5'-CCGG-GCCCTTGTGTGTGACTATAAA-CTCGAG-TTTATAGTCACACACAAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTGTGTGTGACTATAAACTCGAGTTTATAGTCACACACAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTGTGTGTGACTATAAACTCGAGTTTATAGTCACACACAAGGGC-3'

Other clones with same target sequence:

(none)