Construct: shRNA TRCN0000225772

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080817.1-2205s21c1
DNA Barcode:
CATTGCTTTCATGGTCAAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prdm10 (382066)
Gene Description:
PR domain containing 10
Transcript:
RefSeq NM_001080817.1 (CURRENT)
Match location:
Position 2205 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000225772)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTGCTTTCATGGTCAAATA
Hairpin Sequence:
5'-CCGG-CATTGCTTTCATGGTCAAATA-CTCGAG-TATTTGACCATGAAAGCAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTGCTTTCATGGTCAAATACTCGAGTATTTGACCATGAAAGCAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTGCTTTCATGGTCAAATACTCGAGTATTTGACCATGAAAGCAATG-3'

Other clones with same target sequence:

(none)