Construct: shRNA TRCN0000225777

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_620510.3-348s21c1
DNA Barcode:
CGTGAAAGCTTCCCACTTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cers3 (545975)
Gene Description:
ceramide synthase 3
Transcript:
RefSeq XM_620510.3 (NON-CURRENT)
Match location:
Position 348 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000225777)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGAAAGCTTCCCACTTATA
Hairpin Sequence:
5'-CCGG-CGTGAAAGCTTCCCACTTATA-CTCGAG-TATAAGTGGGAAGCTTTCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGAAAGCTTCCCACTTATACTCGAGTATAAGTGGGAAGCTTTCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGAAAGCTTCCCACTTATACTCGAGTATAAGTGGGAAGCTTTCACG-3'

Other clones with same target sequence:

(none)