Construct: shRNA TRCN0000225789

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010703.3-2091s21c1
DNA Barcode:
TGGTCAGCGCGAGACAATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lef1 (16842)
Gene Description:
lymphoid enhancer binding factor 1
Transcript:
RefSeq NM_010703.3 (NON-CURRENT)
Match location:
Position 2091 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000225789)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTCAGCGCGAGACAATTAT
Hairpin Sequence:
5'-CCGG-TGGTCAGCGCGAGACAATTAT-CTCGAG-ATAATTGTCTCGCGCTGACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTCAGCGCGAGACAATTATCTCGAGATAATTGTCTCGCGCTGACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTCAGCGCGAGACAATTATCTCGAGATAATTGTCTCGCGCTGACCA-3'

Other clones with same target sequence:

(none)