Construct: shRNA TRCN0000225811

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011445.3-2593s21c1
DNA Barcode:
TGATCCCAAATCAGACTATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sox6 (20679)
Gene Description:
SRY (sex determining region Y)-box 6
Transcript:
RefSeq NM_011445.3 (NON-CURRENT)
Match location:
Position 2593 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000225811)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATCCCAAATCAGACTATAG
Hairpin Sequence:
5'-CCGG-TGATCCCAAATCAGACTATAG-CTCGAG-CTATAGTCTGATTTGGGATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATCCCAAATCAGACTATAGCTCGAGCTATAGTCTGATTTGGGATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATCCCAAATCAGACTATAGCTCGAGCTATAGTCTGATTTGGGATCA-3'

Other clones with same target sequence:

(none)