Construct: shRNA TRCN0000225897

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081341.1-2859s21c1
DNA Barcode:
TGGCTCCGAGTCACCTTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scaper (244891)
Gene Description:
S phase cyclin A-associated protein in the ER
Transcript:
RefSeq NM_001081341.1 (CURRENT)
Match location:
Position 2859 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000225897)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGCTCCGAGTCACCTTATAA
Hairpin Sequence:
5'-CCGG-TGGCTCCGAGTCACCTTATAA-CTCGAG-TTATAAGGTGACTCGGAGCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGCTCCGAGTCACCTTATAACTCGAGTTATAAGGTGACTCGGAGCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGCTCCGAGTCACCTTATAACTCGAGTTATAAGGTGACTCGGAGCCA-3'

Other clones with same target sequence:

(none)