Construct: shRNA TRCN0000225912
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_001475209.1-2621s21c1
- DNA Barcode:
- CACCCTTGGAGATCGAGTTTG
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC100045900 (100045900)
- Gene Description:
- similar to brain-specific angiogenesis inhibitor 1
- Transcript:
- RefSeq XM_001475209.1 (NON-CURRENT)
- Match location:
- Position 2621 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000225912)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CACCCTTGGAGATCGAGTTTG
- Hairpin Sequence:
-
5'-CCGG-CACCCTTGGAGATCGAGTTTG-CTCGAG-CAAACTCGATCTCCAAGGGTG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCACCCTTGGAGATCGAGTTTGCTCGAGCAAACTCGATCTCCAAGGGTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACACCCTTGGAGATCGAGTTTGCTCGAGCAAACTCGATCTCCAAGGGTG-3'
Other clones with same target sequence:
(none)
