Construct: shRNA TRCN0000225939

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001475896.1-139s21c1
DNA Barcode:
TGTCGTTGGTGTCCCTATCGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC243171 (243171)
Gene Description:
hypothetical LOC243171
Transcript:
RefSeq XM_001475896.1 (NON-CURRENT)
Match location:
Position 139 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000225939)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTCGTTGGTGTCCCTATCGA
Hairpin Sequence:
5'-CCGG-TGTCGTTGGTGTCCCTATCGA-CTCGAG-TCGATAGGGACACCAACGACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCGTTGGTGTCCCTATCGACTCGAGTCGATAGGGACACCAACGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCGTTGGTGTCCCTATCGACTCGAGTCGATAGGGACACCAACGACA-3'

Other clones with same target sequence:

(none)