Construct: shRNA TRCN0000225945

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010906.2-874s21c1
DNA Barcode:
GGAATCCGGACAATCAGATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nfix (18032)
Gene Description:
nuclear factor I/X
Transcript:
RefSeq NM_010906.2 (NON-CURRENT)
Match location:
Position 874 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000225945)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAATCCGGACAATCAGATAG
Hairpin Sequence:
5'-CCGG-GGAATCCGGACAATCAGATAG-CTCGAG-CTATCTGATTGTCCGGATTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAATCCGGACAATCAGATAGCTCGAGCTATCTGATTGTCCGGATTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAATCCGGACAATCAGATAGCTCGAGCTATCTGATTGTCCGGATTCC-3'

Other clones with same target sequence:

  1. TRCN0000234767