Construct: shRNA TRCN0000225998

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081414.1-2203s21c1
DNA Barcode:
CTGTATACAGTTGGGTATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Grm5 (108071)
Gene Description:
glutamate receptor, metabotropic 5
Transcript:
RefSeq NM_001081414.1 (NON-CURRENT)
Match location:
Position 2203 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000225998)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTATACAGTTGGGTATTAT
Hairpin Sequence:
5'-CCGG-CTGTATACAGTTGGGTATTAT-CTCGAG-ATAATACCCAACTGTATACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTATACAGTTGGGTATTATCTCGAGATAATACCCAACTGTATACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTATACAGTTGGGTATTATCTCGAGATAATACCCAACTGTATACAG-3'

Other clones with same target sequence:

  1. TRCN0000219567