Construct: shRNA TRCN0000226013

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_913893.3-35s21c1
DNA Barcode:
CACCAGAGGACAAACAGTTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC638024 (638024)
Gene Description:
similar to AF4/FMR2 family member 3 (LAF-4 protein) (Lymphoid nuclear protein related to AF4)
Transcript:
RefSeq XM_913893.3 (NON-CURRENT)
Match location:
Position 35 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226013)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCAGAGGACAAACAGTTAG
Hairpin Sequence:
5'-CCGG-CACCAGAGGACAAACAGTTAG-CTCGAG-CTAACTGTTTGTCCTCTGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCAGAGGACAAACAGTTAGCTCGAGCTAACTGTTTGTCCTCTGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCAGAGGACAAACAGTTAGCTCGAGCTAACTGTTTGTCCTCTGGTG-3'

Other clones with same target sequence:

(none)