Construct: shRNA TRCN0000226019

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001481337.1-869s21c1
DNA Barcode:
GAGAGTAATCTTAGGATAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm10139 (628676)
Gene Description:
predicted gene 10139
Transcript:
RefSeq XM_001481337.1 (NON-CURRENT)
Match location:
Position 869 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226019)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAGTAATCTTAGGATAATT
Hairpin Sequence:
5'-CCGG-GAGAGTAATCTTAGGATAATT-CTCGAG-AATTATCCTAAGATTACTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAGTAATCTTAGGATAATTCTCGAGAATTATCCTAAGATTACTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAGTAATCTTAGGATAATTCTCGAGAATTATCCTAAGATTACTCTC-3'

Other clones with same target sequence:

(none)