Construct: shRNA TRCN0000226105

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007561.3-3140s21c1
DNA Barcode:
TTCAAGCTTATGGAGTGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bmpr2 (12168)
Gene Description:
bone morphogenetic protein receptor, type II (serine/threonine kinase)
Transcript:
RefSeq NM_007561.3 (NON-CURRENT)
Match location:
Position 3140 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226105)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCAAGCTTATGGAGTGAAAT
Hairpin Sequence:
5'-CCGG-TTCAAGCTTATGGAGTGAAAT-CTCGAG-ATTTCACTCCATAAGCTTGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCAAGCTTATGGAGTGAAATCTCGAGATTTCACTCCATAAGCTTGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCAAGCTTATGGAGTGAAATCTCGAGATTTCACTCCATAAGCTTGAA-3'

Other clones with same target sequence:

(none)