Construct: shRNA TRCN0000226158

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033347.2-3953s21c1
DNA Barcode:
AGTTGGATCAGTCGGCTTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
D430041D05Rik (241589)
Gene Description:
RIKEN cDNA D430041D05 gene
Transcript:
RefSeq NM_001033347.2 (CURRENT)
Match location:
Position 3953 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226158)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTGGATCAGTCGGCTTTAA
Hairpin Sequence:
5'-CCGG-AGTTGGATCAGTCGGCTTTAA-CTCGAG-TTAAAGCCGACTGATCCAACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTGGATCAGTCGGCTTTAACTCGAGTTAAAGCCGACTGATCCAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTGGATCAGTCGGCTTTAACTCGAGTTAAAGCCGACTGATCCAACT-3'

Other clones with same target sequence:

  1. TRCN0000240734