Construct: shRNA TRCN0000226182

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013672.2-589s21c1
DNA Barcode:
CCCTGGAGTAATGCCTAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sp1 (20683)
Gene Description:
trans-acting transcription factor 1
Transcript:
RefSeq NM_013672.2 (CURRENT)
Match location:
Position 589 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226182)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGGAGTAATGCCTAATAT
Hairpin Sequence:
5'-CCGG-CCCTGGAGTAATGCCTAATAT-CTCGAG-ATATTAGGCATTACTCCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGGAGTAATGCCTAATATCTCGAGATATTAGGCATTACTCCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGGAGTAATGCCTAATATCTCGAGATATTAGGCATTACTCCAGGG-3'

Other clones with same target sequence:

(none)