Construct: shRNA TRCN0000226194

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007920.3-1912s21c1
DNA Barcode:
GTTCCAAGTATTAGGACTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Elf1 (13709)
Gene Description:
E74-like factor 1
Transcript:
RefSeq NM_007920.3 (NON-CURRENT)
Match location:
Position 1912 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226194)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCCAAGTATTAGGACTATA
Hairpin Sequence:
5'-CCGG-GTTCCAAGTATTAGGACTATA-CTCGAG-TATAGTCCTAATACTTGGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCCAAGTATTAGGACTATACTCGAGTATAGTCCTAATACTTGGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCCAAGTATTAGGACTATACTCGAGTATAGTCCTAATACTTGGAAC-3'

Other clones with same target sequence:

(none)