Construct: shRNA TRCN0000226232

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080707.1-1333s21c1
DNA Barcode:
ACATGGAAGTGGAGATTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpr155 (68526)
Gene Description:
G protein-coupled receptor 155
Transcript:
RefSeq NM_001080707.1 (NON-CURRENT)
Match location:
Position 1333 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226232)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACATGGAAGTGGAGATTATAA
Hairpin Sequence:
5'-CCGG-ACATGGAAGTGGAGATTATAA-CTCGAG-TTATAATCTCCACTTCCATGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACATGGAAGTGGAGATTATAACTCGAGTTATAATCTCCACTTCCATGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACATGGAAGTGGAGATTATAACTCGAGTTATAATCTCCACTTCCATGT-3'

Other clones with same target sequence:

(none)