Construct: shRNA TRCN0000226238

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_992349.2-1833s21c1
DNA Barcode:
CCTTCAGCCAAAGCTATAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC676710 (676710)
Gene Description:
zinc finger protein 180-like
Transcript:
RefSeq XM_992349.2 (NON-CURRENT)
Match location:
Position 1833 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226238)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCAGCCAAAGCTATAAAC
Hairpin Sequence:
5'-CCGG-CCTTCAGCCAAAGCTATAAAC-CTCGAG-GTTTATAGCTTTGGCTGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCAGCCAAAGCTATAAACCTCGAGGTTTATAGCTTTGGCTGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCAGCCAAAGCTATAAACCTCGAGGTTTATAGCTTTGGCTGAAGG-3'

Other clones with same target sequence:

(none)