Construct: shRNA TRCN0000226281

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020331.2-2317s21c1
DNA Barcode:
GTGAAGCTCTGGGCATCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gtf2ird1 (57080)
Gene Description:
general transcription factor II I repeat domain-containing 1
Transcript:
RefSeq NM_020331.2 (NON-CURRENT)
Match location:
Position 2317 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226281)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAAGCTCTGGGCATCAAAT
Hairpin Sequence:
5'-CCGG-GTGAAGCTCTGGGCATCAAAT-CTCGAG-ATTTGATGCCCAGAGCTTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAAGCTCTGGGCATCAAATCTCGAGATTTGATGCCCAGAGCTTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAAGCTCTGGGCATCAAATCTCGAGATTTGATGCCCAGAGCTTCAC-3'

Other clones with same target sequence:

(none)