Construct: shRNA TRCN0000226283

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015753.3-1031s21c1
DNA Barcode:
CGAAGCGACACGGCCATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zeb2 (24136)
Gene Description:
zinc finger E-box binding homeobox 2
Transcript:
RefSeq NM_015753.3 (NON-CURRENT)
Match location:
Position 1031 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226283)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGCGACACGGCCATTATT
Hairpin Sequence:
5'-CCGG-CGAAGCGACACGGCCATTATT-CTCGAG-AATAATGGCCGTGTCGCTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGCGACACGGCCATTATTCTCGAGAATAATGGCCGTGTCGCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGCGACACGGCCATTATTCTCGAGAATAATGGCCGTGTCGCTTCG-3'

Other clones with same target sequence:

(none)