Construct: shRNA TRCN0000226302

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013673.3-1920s21c1
DNA Barcode:
TCCCGTCTCTAACCTTCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sp100 (20684)
Gene Description:
nuclear antigen Sp100
Transcript:
RefSeq NM_013673.3 (NON-CURRENT)
Match location:
Position 1920 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226302)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCGTCTCTAACCTTCTATA
Hairpin Sequence:
5'-CCGG-TCCCGTCTCTAACCTTCTATA-CTCGAG-TATAGAAGGTTAGAGACGGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCGTCTCTAACCTTCTATACTCGAGTATAGAAGGTTAGAGACGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCGTCTCTAACCTTCTATACTCGAGTATAGAAGGTTAGAGACGGGA-3'

Other clones with same target sequence:

(none)