Construct: shRNA TRCN0000226302
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_013673.3-1920s21c1
- DNA Barcode:
- TCCCGTCTCTAACCTTCTATA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Sp100 (20684)
- Gene Description:
- nuclear antigen Sp100
- Transcript:
- RefSeq NM_013673.3 (NON-CURRENT)
- Match location:
- Position 1920 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000226302)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TCCCGTCTCTAACCTTCTATA
- Hairpin Sequence:
-
5'-CCGG-TCCCGTCTCTAACCTTCTATA-CTCGAG-TATAGAAGGTTAGAGACGGGA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTCCCGTCTCTAACCTTCTATACTCGAGTATAGAAGGTTAGAGACGGGATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATCCCGTCTCTAACCTTCTATACTCGAGTATAGAAGGTTAGAGACGGGA-3'
Other clones with same target sequence:
(none)
