Construct: shRNA TRCN0000226356

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_622716.4-5895s21c1
DNA Barcode:
TATGTCTAGGTTCGCATAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prdm15 (114604)
Gene Description:
PR domain containing 15
Transcript:
RefSeq XM_622716.4 (NON-CURRENT)
Match location:
Position 5895 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226356)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGTCTAGGTTCGCATAAAT
Hairpin Sequence:
5'-CCGG-TATGTCTAGGTTCGCATAAAT-CTCGAG-ATTTATGCGAACCTAGACATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGTCTAGGTTCGCATAAATCTCGAGATTTATGCGAACCTAGACATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGTCTAGGTTCGCATAAATCTCGAGATTTATGCGAACCTAGACATA-3'

Other clones with same target sequence:

(none)