Construct: shRNA TRCN0000226358

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081564.1-317s21c1
DNA Barcode:
AGCTGACATCAGTGACAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm6592 (625480)
Gene Description:
predicted gene 6592
Transcript:
RefSeq NM_001081564.1 (CURRENT)
Match location:
Position 317 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226358)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTGACATCAGTGACAATAA
Hairpin Sequence:
5'-CCGG-AGCTGACATCAGTGACAATAA-CTCGAG-TTATTGTCACTGATGTCAGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTGACATCAGTGACAATAACTCGAGTTATTGTCACTGATGTCAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTGACATCAGTGACAATAACTCGAGTTATTGTCACTGATGTCAGCT-3'

Other clones with same target sequence:

  1. TRCN0000234093