Construct: shRNA TRCN0000226361

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021470.5-646s21c1
DNA Barcode:
CCGCCTTCGGATGCCAAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf32 (56874)
Gene Description:
ring finger protein 32
Transcript:
RefSeq NM_021470.5 (NON-CURRENT)
Match location:
Position 646 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226361)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCCTTCGGATGCCAAATTA
Hairpin Sequence:
5'-CCGG-CCGCCTTCGGATGCCAAATTA-CTCGAG-TAATTTGGCATCCGAAGGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCCTTCGGATGCCAAATTACTCGAGTAATTTGGCATCCGAAGGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCCTTCGGATGCCAAATTACTCGAGTAATTTGGCATCCGAAGGCGG-3'

Other clones with same target sequence:

(none)