Construct: shRNA TRCN0000226387

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001222.2-293s21c1
DNA Barcode:
AGAACGTGAGGCTCGGATATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK2G (818)
Gene Description:
calcium/calmodulin dependent protein kinase II gamma
Transcript:
RefSeq NM_001222.2 (NON-CURRENT)
Match location:
Position 293 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226387)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAACGTGAGGCTCGGATATG
Hairpin Sequence:
5'-CCGG-AGAACGTGAGGCTCGGATATG-CTCGAG-CATATCCGAGCCTCACGTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAACGTGAGGCTCGGATATGCTCGAGCATATCCGAGCCTCACGTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAACGTGAGGCTCGGATATGCTCGAGCATATCCGAGCCTCACGTTCT-3'

Other clones with same target sequence:

(none)