Construct: shRNA TRCN0000226412

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002713.2-1272s21c1
DNA Barcode:
TATAACCCTGAAGCTGTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP1R8 (5511)
Gene Description:
protein phosphatase 1 regulatory subunit 8
Transcript:
RefSeq NM_002713.2 (NON-CURRENT)
Match location:
Position 1272 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226412)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATAACCCTGAAGCTGTAAAT
Hairpin Sequence:
5'-CCGG-TATAACCCTGAAGCTGTAAAT-CTCGAG-ATTTACAGCTTCAGGGTTATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATAACCCTGAAGCTGTAAATCTCGAGATTTACAGCTTCAGGGTTATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATAACCCTGAAGCTGTAAATCTCGAGATTTACAGCTTCAGGGTTATA-3'

Other clones with same target sequence:

(none)