Construct: shRNA TRCN0000226438

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017593.3-760s21c1
DNA Barcode:
CTTTGGCAGTGCCACTAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BMP2K (55589)
Gene Description:
BMP2 inducible kinase
Transcript:
RefSeq NM_017593.3 (NON-CURRENT)
Match location:
Position 760 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000226438)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGGCAGTGCCACTAATAA
Hairpin Sequence:
5'-CCGG-CTTTGGCAGTGCCACTAATAA-CTCGAG-TTATTAGTGGCACTGCCAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGGCAGTGCCACTAATAACTCGAGTTATTAGTGGCACTGCCAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGGCAGTGCCACTAATAACTCGAGTTATTAGTGGCACTGCCAAAG-3'

Other clones with same target sequence:

(none)