Construct: shRNA TRCN0000229313

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001249.1-382s21c1
DNA Barcode:
AGCACCTTGTATGGAATTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ENTPD5 (957)
Gene Description:
ectonucleoside triphosphate diphosphohydrolase 5 (inactive)
Transcript:
RefSeq NM_001249.1 (NON-CURRENT)
Match location:
Position 382 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229313)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCACCTTGTATGGAATTATG
Hairpin Sequence:
5'-CCGG-AGCACCTTGTATGGAATTATG-CTCGAG-CATAATTCCATACAAGGTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCACCTTGTATGGAATTATGCTCGAGCATAATTCCATACAAGGTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCACCTTGTATGGAATTATGCTCGAGCATAATTCCATACAAGGTGCT-3'

Other clones with same target sequence:

(none)