Construct: shRNA TRCN0000229323

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006290.2-1085s21c1
DNA Barcode:
ATCTGGTAGATGATTACTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNFAIP3 (7128)
Gene Description:
TNF alpha induced protein 3
Transcript:
RefSeq NM_006290.2 (NON-CURRENT)
Match location:
Position 1085 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229323)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCTGGTAGATGATTACTTTG
Hairpin Sequence:
5'-CCGG-ATCTGGTAGATGATTACTTTG-CTCGAG-CAAAGTAATCATCTACCAGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCTGGTAGATGATTACTTTGCTCGAGCAAAGTAATCATCTACCAGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCTGGTAGATGATTACTTTGCTCGAGCAAAGTAATCATCTACCAGAT-3'

Other clones with same target sequence:

(none)