Construct: shRNA TRCN0000229335

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032493.2-271s21c1
DNA Barcode:
TCCGTTTCATGTGGATCAAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP1M1 (8907)
Gene Description:
adaptor related protein complex 1 subunit mu 1
Transcript:
RefSeq NM_032493.2 (NON-CURRENT)
Match location:
Position 271 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229335)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCGTTTCATGTGGATCAAAC
Hairpin Sequence:
5'-CCGG-TCCGTTTCATGTGGATCAAAC-CTCGAG-GTTTGATCCACATGAAACGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCGTTTCATGTGGATCAAACCTCGAGGTTTGATCCACATGAAACGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCGTTTCATGTGGATCAAACCTCGAGGTTTGATCCACATGAAACGGA-3'

Other clones with same target sequence:

(none)