Construct: shRNA TRCN0000229361

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182896.1-2051s21c1
DNA Barcode:
CATCGTAATTTGACCTAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARL13B (200894)
Gene Description:
ADP ribosylation factor like GTPase 13B
Transcript:
RefSeq NM_182896.1 (NON-CURRENT)
Match location:
Position 2051 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229361)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGTAATTTGACCTAATTT
Hairpin Sequence:
5'-CCGG-CATCGTAATTTGACCTAATTT-CTCGAG-AAATTAGGTCAAATTACGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGTAATTTGACCTAATTTCTCGAGAAATTAGGTCAAATTACGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGTAATTTGACCTAATTTCTCGAGAAATTAGGTCAAATTACGATG-3'

Other clones with same target sequence:

(none)