Construct: shRNA TRCN0000229384

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012067.2-302s21c1
DNA Barcode:
TACCAAGGCCATTCCACTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKR7A3 (22977)
Gene Description:
aldo-keto reductase family 7 member A3
Transcript:
RefSeq NM_012067.2 (NON-CURRENT)
Match location:
Position 302 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229384)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACCAAGGCCATTCCACTGTT
Hairpin Sequence:
5'-CCGG-TACCAAGGCCATTCCACTGTT-CTCGAG-AACAGTGGAATGGCCTTGGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACCAAGGCCATTCCACTGTTCTCGAGAACAGTGGAATGGCCTTGGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACCAAGGCCATTCCACTGTTCTCGAGAACAGTGGAATGGCCTTGGTA-3'

Other clones with same target sequence:

(none)