Construct: shRNA TRCN0000229438

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008228.2-69s21c1
DNA Barcode:
GATGTTGGAAACTACTATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hdac1 (433759)
Gene Description:
histone deacetylase 1
Transcript:
RefSeq NM_008228.2 (CURRENT)
Match location:
Position 69 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229438)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGTTGGAAACTACTATTAT
Hairpin Sequence:
5'-CCGG-GATGTTGGAAACTACTATTAT-CTCGAG-ATAATAGTAGTTTCCAACATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGTTGGAAACTACTATTATCTCGAGATAATAGTAGTTTCCAACATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGTTGGAAACTACTATTATCTCGAGATAATAGTAGTTTCCAACATC-3'

Other clones with same target sequence:

(none)