Construct: shRNA TRCN0000229449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031198.2-417s21c1
DNA Barcode:
GTGCTGAGAAATGGCATTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tfec (21426)
Gene Description:
transcription factor EC
Transcript:
RefSeq NM_031198.2 (NON-CURRENT)
Match location:
Position 417 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCTGAGAAATGGCATTTAT
Hairpin Sequence:
5'-CCGG-GTGCTGAGAAATGGCATTTAT-CTCGAG-ATAAATGCCATTTCTCAGCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCTGAGAAATGGCATTTATCTCGAGATAAATGCCATTTCTCAGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCTGAGAAATGGCATTTATCTCGAGATAAATGCCATTTCTCAGCAC-3'

Other clones with same target sequence:

  1. TRCN0000085610