Construct: shRNA TRCN0000229527

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010591.2-1929s21c1
DNA Barcode:
GCTAACGCAGCAGTTGCAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Jun (16476)
Gene Description:
jun proto-oncogene
Transcript:
RefSeq NM_010591.2 (CURRENT)
Match location:
Position 1929 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229527)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAACGCAGCAGTTGCAAAC
Hairpin Sequence:
5'-CCGG-GCTAACGCAGCAGTTGCAAAC-CTCGAG-GTTTGCAACTGCTGCGTTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAACGCAGCAGTTGCAAACCTCGAGGTTTGCAACTGCTGCGTTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAACGCAGCAGTTGCAAACCTCGAGGTTTGCAACTGCTGCGTTAGC-3'

Other clones with same target sequence:

  1. TRCN0000338221