Construct: shRNA TRCN0000229576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033820.3-696s21c1
DNA Barcode:
TATTCGTGAACAGCCTTATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp551 (619331)
Gene Description:
zinc fingr protein 551
Transcript:
RefSeq NM_001033820.3 (CURRENT)
Match location:
Position 696 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATTCGTGAACAGCCTTATAG
Hairpin Sequence:
5'-CCGG-TATTCGTGAACAGCCTTATAG-CTCGAG-CTATAAGGCTGTTCACGAATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATTCGTGAACAGCCTTATAGCTCGAGCTATAAGGCTGTTCACGAATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATTCGTGAACAGCCTTATAGCTCGAGCTATAAGGCTGTTCACGAATA-3'

Other clones with same target sequence:

(none)