Construct: shRNA TRCN0000229580

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008391.4-391s21c1
DNA Barcode:
CATCAACCAGGAATAGATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Irf2 (16363)
Gene Description:
interferon regulatory factor 2
Transcript:
RefSeq NM_008391.4 (CURRENT)
Match location:
Position 391 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229580)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCAACCAGGAATAGATAAA
Hairpin Sequence:
5'-CCGG-CATCAACCAGGAATAGATAAA-CTCGAG-TTTATCTATTCCTGGTTGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCAACCAGGAATAGATAAACTCGAGTTTATCTATTCCTGGTTGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCAACCAGGAATAGATAAACTCGAGTTTATCTATTCCTGGTTGATG-3'

Other clones with same target sequence:

(none)