Construct: shRNA TRCN0000229600

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011084.2-3314s21c1
DNA Barcode:
CACACCTTAAGTCTTACTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pik3c2g (18705)
Gene Description:
phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 gamma
Transcript:
RefSeq NM_011084.2 (CURRENT)
Match location:
Position 3314 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229600)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACACCTTAAGTCTTACTAAT
Hairpin Sequence:
5'-CCGG-CACACCTTAAGTCTTACTAAT-CTCGAG-ATTAGTAAGACTTAAGGTGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACACCTTAAGTCTTACTAATCTCGAGATTAGTAAGACTTAAGGTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACACCTTAAGTCTTACTAATCTCGAGATTAGTAAGACTTAAGGTGTG-3'

Other clones with same target sequence:

(none)