Construct: shRNA TRCN0000229646

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_078483.2-759s21c1
DNA Barcode:
ACAACAATGAGACGGTGATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC36A1 (206358)
Gene Description:
solute carrier family 36 member 1
Transcript:
RefSeq NM_078483.2 (NON-CURRENT)
Match location:
Position 759 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229646)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAACAATGAGACGGTGATTC
Hairpin Sequence:
5'-CCGG-ACAACAATGAGACGGTGATTC-CTCGAG-GAATCACCGTCTCATTGTTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAACAATGAGACGGTGATTCCTCGAGGAATCACCGTCTCATTGTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAACAATGAGACGGTGATTCCTCGAGGAATCACCGTCTCATTGTTGT-3'

Other clones with same target sequence:

(none)