Construct: shRNA TRCN0000229705

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007031.1-956s21c1
DNA Barcode:
GGTGCTCTTGGACACCATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HSF2BP (11077)
Gene Description:
heat shock transcription factor 2 binding protein
Transcript:
RefSeq NM_007031.1 (NON-CURRENT)
Match location:
Position 956 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229705)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTGCTCTTGGACACCATATT
Hairpin Sequence:
5'-CCGG-GGTGCTCTTGGACACCATATT-CTCGAG-AATATGGTGTCCAAGAGCACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTGCTCTTGGACACCATATTCTCGAGAATATGGTGTCCAAGAGCACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTGCTCTTGGACACCATATTCTCGAGAATATGGTGTCCAAGAGCACC-3'

Other clones with same target sequence:

(none)