Construct: shRNA TRCN0000229788
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_015440.3-2801s21c1
- DNA Barcode:
- GTGAGAGAGGCTGCGAGTAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MTHFD1L (25902)
- Gene Description:
- methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like
- Transcript:
- RefSeq NM_015440.3 (NON-CURRENT)
- Match location:
- Position 2801 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000229788)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GTGAGAGAGGCTGCGAGTAAA
- Hairpin Sequence:
-
5'-CCGG-GTGAGAGAGGCTGCGAGTAAA-CTCGAG-TTTACTCGCAGCCTCTCTCAC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGTGAGAGAGGCTGCGAGTAAACTCGAGTTTACTCGCAGCCTCTCTCACTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGTGAGAGAGGCTGCGAGTAAACTCGAGTTTACTCGCAGCCTCTCTCAC-3'
Other clones with same target sequence:
(none)
