Construct: shRNA TRCN0000229839

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018332.3-165s21c1
DNA Barcode:
GCAGATACCAATGGTATTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDX19A (55308)
Gene Description:
DEAD-box helicase 19A
Transcript:
RefSeq NM_018332.3 (NON-CURRENT)
Match location:
Position 165 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229839)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATACCAATGGTATTATC
Hairpin Sequence:
5'-CCGG-GCAGATACCAATGGTATTATC-CTCGAG-GATAATACCATTGGTATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATACCAATGGTATTATCCTCGAGGATAATACCATTGGTATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATACCAATGGTATTATCCTCGAGGATAATACCATTGGTATCTGC-3'

Other clones with same target sequence:

(none)